Fasting is not necessary.
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It is recommended to do the lab test first thing in the morning, right after you wake up before a meal.

However, if this is for your follow-up labs, it should be:

ENCLOMIPHENE/PREGNENOLONE

Do the test an HOUR (1 HOUR) after you take your medication.

ORAL TRT+

Do the test 4 HOURS after you take your medication, with at least 30g of fat, ideally from whole foods, not oils.

WEIGHT LOSS

You can perform the test at any time, making sure you are hydrated before completing the test. (This goes for baseline and/or follow ups)